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hcf medium  (PromoCell)


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    PromoCell hcf medium
    Hcf Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hcf+medium/Fibroblast+Growth+Medium+3/pmc12611762-526-6-13
    Average 95 stars, based on 30 article reviews
    hcf medium - by Bioz Stars, 2026-11
    95/100 stars

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    Centrifugation:

    Article Title: Spatiotemporal dynamics of the cardioimmune niche during lesion repair
    Article Snippet: Human cardiac fibroblasts (HCFs) were purchased from Promocell (C-12375), having been isolated from the ventricles of adult human hearts and cryopreserved.Human cardiac fibroblasts (HCFs) were purchased from Promocell (C-12375), having been isolated from the ventricles of adult human hearts and cryopreserved.. Cells were defrosted, resuspended in full HCF medium (Growth Medium 3 with supplements, Promocell, C-23025) and allowed to grow in a T75 flask (Merck, C7231-120EA) until 80% confluence.. HCFs were then trypsinized using 3 ml of trypsin–EDTA (0.25%) with phenol red (Gibco, 25200072) for 5 min at 37 °C, followed by centrifugation (300 g , 5 min, 4 °C) and pellet resuspension by HCF medium.HCFs were then trypsinized using 3 ml of trypsin–EDTA (0.25%) with phenol red (Gibco, 25200072) for 5 min at 37 °C, followed by centrifugation (300 g , 5 min, 4 °C) and pellet resuspension by HCF medium.

    Article Title: Spatiotemporal dynamics of the cardioimmune niche during lesion repair.
    Article Snippet: Human primary cardiac FB culturing Human cardiac fibroblasts (HCFs) were purchased from Promocell (C-12375), having been isolated from the ventricles of adult human hearts and cryopreserved.Human primary cardiac FB culturing Human cardiac fibroblasts (HCFs) were purchased from Promocell (C-12375), having been isolated from the ventricles of adult human hearts and cryopreserved.. Cells were defrosted, resuspended in full HCF medium (Growth Medium 3 with supplements, Promocell, C-23025) and allowed to grow in a T75 flask (Merck, C7231-120EA) until 80% confluence.. HCFs were then trypsinized using 3 ml of trypsin–EDTA (0.25%) with phenol red (Gibco, 25200072) for 5 min at 37 °C, followed Nature Cardiovascular Research Article https://doi.org/10.1038/s44161-025-00739-6 by centrifugation (300g, 5 min, 4 °C) and pellet resuspension by HCF medium.HCFs were then trypsinized using 3 ml of trypsin–EDTA (0.25%) with phenol red (Gibco, 25200072) for 5 min at 37 °C, followed Nature Cardiovascular Research Article https://doi.org/10.1038/s44161-025-00739-6 by centrifugation (300g, 5 min, 4 °C) and pellet resuspension by HCF medium.

    Article Title: Spatio-temporal dynamics of the fibrotic niche in cardiac repair
    Article Snippet: cardiac fibroblasts (HCF) were purchased from Promocell (C-12375), having been isolated from the ventricles of adult human hearts and cryopreserved. .. Cells were defrosted, resuspended in full HCF medium (Growth Medium 3 with supplements, Promocell, C-23025), and allowed to grow in a T75 flask (Merck, C7231-120EA) until 80% confluence. .. HCF were then trypsinized using 3mL of Trypsin-EDTA (0,25%) with phenol red (Gibco, 25200072) for 5 minutes at 37°C, followed by centrifugation (300 g



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    Image Search Results


    Fibroblast-derived cytokine and chemokine promote iCM survival after erastin treatment (A) Flowchart of conditioned media and cytokine array experiment. (B) Survival rate of iCMs, cultured in control medium (DMEM), HCF conditioned medium, or H 2 O 2 -treated HCF conditioned medium, after erastin treatment, normalized to respective DMSO control groups. (C) Cytokine-chemokine protein array blotting image of conditioned media from vehicle- or H 2 O 2 -treated HCFs. (D) Blotting signal intensity of IL-8 and EGF. (E and F) Survival rate of iCMs after erastin treatment in the presence of IL-8 (E) or EGF (F), normalized to DMSO control groups. (G) qPCR of CXCR1 and CXCR2 in total blood cells and purified cardiomyocyte nuclei after P1 LAD-O. (H) Western blot of CXCR1, CXCR2, and α-tubulin in human iCMs after DMSO or erastin treatment. (I) Normalized band (immuno-blotting) intensity of CXCR1 and CXCR2 in (H). All bar graphs represent mean ± SD. ∗p < 0.05; ∗∗p < 0.01; NS, not significant by t test.

    Journal: iScience

    Article Title: Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury

    doi: 10.1016/j.isci.2024.109219

    Figure Lengend Snippet: Fibroblast-derived cytokine and chemokine promote iCM survival after erastin treatment (A) Flowchart of conditioned media and cytokine array experiment. (B) Survival rate of iCMs, cultured in control medium (DMEM), HCF conditioned medium, or H 2 O 2 -treated HCF conditioned medium, after erastin treatment, normalized to respective DMSO control groups. (C) Cytokine-chemokine protein array blotting image of conditioned media from vehicle- or H 2 O 2 -treated HCFs. (D) Blotting signal intensity of IL-8 and EGF. (E and F) Survival rate of iCMs after erastin treatment in the presence of IL-8 (E) or EGF (F), normalized to DMSO control groups. (G) qPCR of CXCR1 and CXCR2 in total blood cells and purified cardiomyocyte nuclei after P1 LAD-O. (H) Western blot of CXCR1, CXCR2, and α-tubulin in human iCMs after DMSO or erastin treatment. (I) Normalized band (immuno-blotting) intensity of CXCR1 and CXCR2 in (H). All bar graphs represent mean ± SD. ∗p < 0.05; ∗∗p < 0.01; NS, not significant by t test.

    Article Snippet: Human Cardiac Fibroblasts (HCF) (Cell Applications Inc, 306V-05a) were cultured in HCF Growth Medium (Cell Applications Inc, 316–500).

    Techniques: Derivative Assay, Cell Culture, Control, Protein Array, Purification, Western Blot

    Cardiac fibroblasts interact with cardiomyocytes through gap junctions to share free iron (A and B) Wild-type mouse heart tissue stained for Fth1 [magenta, (A)] or Ftl [magenta, (B)], with cTnT (green) and DAPI (blue) at 1DPMI after P7 LAD-O. Arrows: non-cardiomyocytes positive for Fth1 (A) or Ftl (B). (C and D) Mouse heart tissue stained for Fth1 [red, (C)] or Ftl [red, (D)], with Pdgfrα (gray), cTnT (green), and DAPI (blue) at 1 DPMI after P7 LAD-O. Arrows: cells positive for Pdgfrα and Fth1 (C) or Ftl (D). (E and F) Mouse heart tissue stained for Fth1 [green, (E)] or Ftl [green, (F)], with Pdgfrα (red), MF20 (gray), and DAPI (blue) at 6 DPMI after P7 LAD-O. Arrows: cells positive for Pdgfrα and Fth1 (E) or Ftl (F). (G) Diagram of cardiomyocyte-fibroblast interaction after MI. (H and I) Mouse heart section stained for Cx45 [green, (H)] or Cx43 [green, (I)], with Pdgfrα (red), MF20 (gray), and DAPI (blue) after P7 LAD-O. Arrows: potential locations of gap junctions between cardiomyocytes and fibroblasts. (J–L) Co-cultured iCM and HCF stained for VIMENTIN (VIM, gray), free Fe 2+ (red), DAPI (blue), and imaged with TITIN-GFP (green) after DMSO (J) or erastin (15 μM) (K, L) treatment. Asterisks: HCFs with accumulation of Fe 2+ . (M) siRNA knockdown of CX43 and CX45 simultaneously in iCM-HCF co-culture; Fe 2+ fluorescent intensity ratio of iCMs over HCFs was quantified after erastin or DMSO treatment. LV, left ventricle. All bar graphs represent mean ± SD. ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001 by t test. Scale bar, 75 μm (A, B, E, F, H), 25 μm (C, D, I, J–L). See also <xref ref-type=Figures S4 and . " width="100%" height="100%">

    Journal: iScience

    Article Title: Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury

    doi: 10.1016/j.isci.2024.109219

    Figure Lengend Snippet: Cardiac fibroblasts interact with cardiomyocytes through gap junctions to share free iron (A and B) Wild-type mouse heart tissue stained for Fth1 [magenta, (A)] or Ftl [magenta, (B)], with cTnT (green) and DAPI (blue) at 1DPMI after P7 LAD-O. Arrows: non-cardiomyocytes positive for Fth1 (A) or Ftl (B). (C and D) Mouse heart tissue stained for Fth1 [red, (C)] or Ftl [red, (D)], with Pdgfrα (gray), cTnT (green), and DAPI (blue) at 1 DPMI after P7 LAD-O. Arrows: cells positive for Pdgfrα and Fth1 (C) or Ftl (D). (E and F) Mouse heart tissue stained for Fth1 [green, (E)] or Ftl [green, (F)], with Pdgfrα (red), MF20 (gray), and DAPI (blue) at 6 DPMI after P7 LAD-O. Arrows: cells positive for Pdgfrα and Fth1 (E) or Ftl (F). (G) Diagram of cardiomyocyte-fibroblast interaction after MI. (H and I) Mouse heart section stained for Cx45 [green, (H)] or Cx43 [green, (I)], with Pdgfrα (red), MF20 (gray), and DAPI (blue) after P7 LAD-O. Arrows: potential locations of gap junctions between cardiomyocytes and fibroblasts. (J–L) Co-cultured iCM and HCF stained for VIMENTIN (VIM, gray), free Fe 2+ (red), DAPI (blue), and imaged with TITIN-GFP (green) after DMSO (J) or erastin (15 μM) (K, L) treatment. Asterisks: HCFs with accumulation of Fe 2+ . (M) siRNA knockdown of CX43 and CX45 simultaneously in iCM-HCF co-culture; Fe 2+ fluorescent intensity ratio of iCMs over HCFs was quantified after erastin or DMSO treatment. LV, left ventricle. All bar graphs represent mean ± SD. ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001 by t test. Scale bar, 75 μm (A, B, E, F, H), 25 μm (C, D, I, J–L). See also Figures S4 and .

    Article Snippet: Human Cardiac Fibroblasts (HCF) (Cell Applications Inc, 306V-05a) were cultured in HCF Growth Medium (Cell Applications Inc, 316–500).

    Techniques: Staining, Cell Culture, Knockdown, Co-Culture Assay

    Journal: iScience

    Article Title: Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury

    doi: 10.1016/j.isci.2024.109219

    Figure Lengend Snippet:

    Article Snippet: Human Cardiac Fibroblasts (HCF) (Cell Applications Inc, 306V-05a) were cultured in HCF Growth Medium (Cell Applications Inc, 316–500).

    Techniques: Recombinant, Produced, Virus, Bicinchoninic Acid Protein Assay, Purification, Sequencing, Negative Control, Software