hcf medium  (PromoCell)

Journal: iScience

Article Title: Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury

doi: 10.1016/j.isci.2024.109219

Figure Lengend Snippet: Fibroblast-derived cytokine and chemokine promote iCM survival after erastin treatment (A) Flowchart of conditioned media and cytokine array experiment. (B) Survival rate of iCMs, cultured in control medium (DMEM), HCF conditioned medium, or H 2 O 2 -treated HCF conditioned medium, after erastin treatment, normalized to respective DMSO control groups. (C) Cytokine-chemokine protein array blotting image of conditioned media from vehicle- or H 2 O 2 -treated HCFs. (D) Blotting signal intensity of IL-8 and EGF. (E and F) Survival rate of iCMs after erastin treatment in the presence of IL-8 (E) or EGF (F), normalized to DMSO control groups. (G) qPCR of CXCR1 and CXCR2 in total blood cells and purified cardiomyocyte nuclei after P1 LAD-O. (H) Western blot of CXCR1, CXCR2, and α-tubulin in human iCMs after DMSO or erastin treatment. (I) Normalized band (immuno-blotting) intensity of CXCR1 and CXCR2 in (H). All bar graphs represent mean ± SD. ∗p < 0.05; ∗∗p < 0.01; NS, not significant by t test.

Article Snippet: Human Cardiac Fibroblasts (HCF) (Cell Applications Inc, 306V-05a) were cultured in HCF Growth Medium (Cell Applications Inc, 316–500).

Techniques: Derivative Assay, Cell Culture, Control, Protein Array, Purification, Western Blot

Journal: iScience

Article Title: Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury

doi: 10.1016/j.isci.2024.109219

Figure Lengend Snippet: Cardiac fibroblasts interact with cardiomyocytes through gap junctions to share free iron (A and B) Wild-type mouse heart tissue stained for Fth1 [magenta, (A)] or Ftl [magenta, (B)], with cTnT (green) and DAPI (blue) at 1DPMI after P7 LAD-O. Arrows: non-cardiomyocytes positive for Fth1 (A) or Ftl (B). (C and D) Mouse heart tissue stained for Fth1 [red, (C)] or Ftl [red, (D)], with Pdgfrα (gray), cTnT (green), and DAPI (blue) at 1 DPMI after P7 LAD-O. Arrows: cells positive for Pdgfrα and Fth1 (C) or Ftl (D). (E and F) Mouse heart tissue stained for Fth1 [green, (E)] or Ftl [green, (F)], with Pdgfrα (red), MF20 (gray), and DAPI (blue) at 6 DPMI after P7 LAD-O. Arrows: cells positive for Pdgfrα and Fth1 (E) or Ftl (F). (G) Diagram of cardiomyocyte-fibroblast interaction after MI. (H and I) Mouse heart section stained for Cx45 [green, (H)] or Cx43 [green, (I)], with Pdgfrα (red), MF20 (gray), and DAPI (blue) after P7 LAD-O. Arrows: potential locations of gap junctions between cardiomyocytes and fibroblasts. (J–L) Co-cultured iCM and HCF stained for VIMENTIN (VIM, gray), free Fe 2+ (red), DAPI (blue), and imaged with TITIN-GFP (green) after DMSO (J) or erastin (15 μM) (K, L) treatment. Asterisks: HCFs with accumulation of Fe 2+ . (M) siRNA knockdown of CX43 and CX45 simultaneously in iCM-HCF co-culture; Fe 2+ fluorescent intensity ratio of iCMs over HCFs was quantified after erastin or DMSO treatment. LV, left ventricle. All bar graphs represent mean ± SD. ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001 by t test. Scale bar, 75 μm (A, B, E, F, H), 25 μm (C, D, I, J–L). See also Figures S4 and .

Article Snippet: Human Cardiac Fibroblasts (HCF) (Cell Applications Inc, 306V-05a) were cultured in HCF Growth Medium (Cell Applications Inc, 316–500).

Techniques: Staining, Cell Culture, Knockdown, Co-Culture Assay

Journal: iScience

Article Title: Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury

doi: 10.1016/j.isci.2024.109219

Figure Lengend Snippet:

Article Snippet: Human Cardiac Fibroblasts (HCF) (Cell Applications Inc, 306V-05a) were cultured in HCF Growth Medium (Cell Applications Inc, 316–500).

Techniques: Recombinant, Produced, Virus, Bicinchoninic Acid Protein Assay, Purification, Sequencing, Negative Control, Software